Local Flower-shop as a decentralized data-centre
On Site Lab
Our methods to store data in Plants:
Method 1: Data Injection
Using a syringe take in the sequenced DNA solution
Take plant and apply solution to the underside of a leaf
Allow leaf veins to absorb the solution
Simple and fast
Convenient if looking for short term data usage
Transient Expression, no incorporation of the exogenous DNA, most is degraded before integration.
Method nr.2: Floral dip
Floral dip transformation is notable for a number of reasons. First, it is strikingly simple to perform.
Agrobacterium is applied to flowering plants
Wait for the plant to reach full maturity and germinate
Transgenic plants are then selected among the progeny seedlings.
No need for plant regeneration
Avoid somaclonal variation
Method nr.3: Biolistic Shot (microprojectile bombardment)
Microscopic gold particles are used as ’bullets’ to deliver DNA into callus cells. The gold particles are coated with hundreds of copies of the data of interest. Once inside the nucleus of a cell, the genes dissolve off of the gold particle and can potentially insert into a chromosome.
Stable expression: some incorporation of exogenous DNA into plant genome
Longer lasting data
Expression of transgene through life cycle of plant
Requires special training
Several copies of the data will often insert into a chromosomal position, and can be detected as DNA that should not be expressed by the plant cell, therefore copies are silenced.
Our data florist advised clients on how to care for their plant, to ensure their data could bloom and grow. We created a data-care card that lived with the plant, providing plant information and care instructions. Each visitor left with an encoded plant and a special download kit, allowing them to send us a sample of their plant, which we could use to read their data back when required.
Growing data in Thailand
Educational posters designed by: Kuba Bogacki, Krzysztof Seyfried, Olek Znosko
Project Advisors: Cecilie Ida Cetti Hansen, Johan Andersen-Ranberg, Martin Malthe Borch