Local Flowershop as a decentralized Grow Your Own CLoud data-centre experience
We used data prescriptions to explore people’s data requirements while educating them on the possibilities of using DNA based data storage, such as storage capacity, ultra-longevity and the ability to cross-pollinate or re-plant data.
The on-site lab, where a data scientist was on hand to demonstrate three laboratory techniques that could be used to encode synthetic DNA containing data, to an organism like a plant.
Our methods to store data in Plants:
Method 1: Data Injection
Using a syringe take in the sequenced DNA solution
Take plant and apply solution to the underside of a leaf
Allow leaf veins to absorb the solution
Simple and fast
Convenient if looking for short term data usage
Transient Expression, no incorporation of the exogenous DNA, most is degraded before integration.
Method nr.2: Floral dip
Floral dip transformation is notable for a number of reasons. First, it is strikingly simple to perform.
Agrobacterium is applied to flowering plants
Wait for the plant to reach full maturity and germinate
Transgenic plants are then selected among the progeny seedlings.
No need for plant regeneration
Avoid somaclonal variation
Method nr.3: Biolistic Shot (microprojectile bombardment)
Microscopic gold particles are used as ’bullets’ to deliver DNA into callus cells. The gold particles are coated with hundreds of copies of the data of interest. Once inside the nucleus of a cell, the genes dissolve off of the gold particle and can potentially insert into a chromosome.
Stable expression: some incorporation of exogenous DNA into plant genome
Longer lasting data
Expression of transgene through life cycle of plant
Requires special training
Several copies of the data will often insert into a chromosomal position, and can be detected as DNA that should not be expressed by the plant cell, therefore copies are silenced.
Speculative story about Growing data in Thailand